In collaboration with Don Rio’s lab at UC Berkeley, we report a 3.6 Å resolution cryo-EM structure of the P element transposase strand-transfer (i.e. transpososome) complex. Not only did we encounter severe preferential orientation issues (which we overcame with tilting), we had to build the entire structure (nearly 900 amino acids) de novo!
https://www.nature.com/articles/s41594-019-0319-6
See the accompanying (and excellent!) news&views written by Phoebe Rice.
